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KMID : 0620920090410090629
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Experimental & Molecular Medicine
2009 Volume.41 No. 9 p.629 ~ p.637
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Differential regulation of inducible nitric oxide synthase and cyclooxygenase-2 expression by superoxide dismutase in lipopolysaccharide stimulated RAW 264.7 cells
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Lee Ji-Ae
Song Ha-Yong
Ju Sung-Mi
Lee Su-Jin
Kwon Hyung-Joo
Eum Won-Sik
Jang Sang-Ho
Choi Soo-Young
Park Jin-Seu
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Abstract
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Inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) have been known to be involved in various pathophysiological processes such as inflammation. This study was performed to determine the regulatory function of superoxide dismutase (SOD) on the LPS-induced expression of iNOS, and COX-2 in RAW 264.7 cells. When a cell-permeable SOD, Tat- SOD, was added to the culture medium of RAW 264.7 cells, it rapidly entered the cells in a dose-dependent manner. Treatment of RAW 264.7 cells with Tat-SOD led to decrease in LPS-induced ROS generation. Pretreatment with Tat-SOD significantly inhibited LPS-induced expression of iNOS and NO production but had no effect on the expression of COX-2 and PGE2 production in RAW 264.7 cells. Tat-SOD inhibited LPS-induced NF-κB DNA binding activity, IκBα degradation and activation of MAP kinases. These data suggest that SOD differentially regulate expression of iNOS and COX-2 in LPS-stimulated RAW 264.7 cells.
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KEYWORD
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cyclooxygenase 2, lipopolysaccharides, NF-κB, nitric oxide synthase type II, reactive oxygen species, superoxide dismutase
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